Splice variants - alternative splicing may create different sized proteins produced from the same geneĤ. many proteins are synthesized as pro-proteins and then cleaved to give the active form, e.g. phosphorylation, glycosylation etc, which increases the size of the proteinĢ. However, migration is also affected by other factors, so the actual band size observed may differ from that predicted. In general, the smaller the protein, the faster it migrates through the gel. Western blotting is a technique that separates proteins based on size. 13 Proteolytic breakdown of the antigen.9 Excess signal multiple bands/too much banding.5.2 Uneven band size in lanes probed for the same protein.5.1 Smile effect of the bands/Dumbbell shaped bands.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |